卡介苗活化家兔肺泡巨噬细胞和腹腔巨噬细胞定量细胞化学比较研究

COMPARATVE STUDY OF QUANTITATIVE CYTOCHEMISTRY ON THE BCG ACTIVATED ALVEOLAR MACROPHAGES WITH THAT OF PERITONEAL MACROPHAGES

本实验测定了卡介苗免疫后家兔肺泡巨噬细胞和腹腔巨噬细胞内几种酶活性。结果表明肺泡巨噬细胞内ACP、SDH、NSE含量高于腹腔巨噬细胞:而LDH低于腹腔巨噬细胞。卡介苗活化后两种巨噬细胞的ACP、SDH、NSE含量有明显增加。肺泡巨噬细胞的LDH也有明显增加,而腹腔巨噬细胞LDH则变化不明显。结果证实卡介苗活化提高了巨噬细胞的杀伤能力,但未改变其代谢途径。本实验为活化巨噬细胞的进一步研究提供了细胞化学基础。

Some intracellular enzyme-activities of BCG-activated alveolar macrophages (AM) and peritoneal macrophages (PM) were studied. It was found that (in normal) the activities of acid phosphatas (ACP), succinate dehydrogenase (SDH), non-specific esterase (NSE) in AM were higher than those in PM, but that of lactate dehydrogenase (LDH) was lower. Both in BCG activated AM and PM, the activities of ACP, SDH, NSE increased significantly, and that of LDH also enhanced in activated AM but not in PM.