农药污染区域细菌质粒分布的调查

SURVEY OF BACTERIAL PLASMID DISTRIBUTION IN AREAS POLLUTED BY PESTICIDE

从华北(大沽)、华中(武汉)、华南(柳州)三个不同地理位置受农药(PCP)污染的土壤和水中分离出144株细菌。用琼脂糖凝胶电泳技术进行质粒检测,105株有质粒,占检测总菌数的72%,而非污染的对照区采样分离的菌株中只有3%有质粒。大多数质粒分子量在50—100Md范围。携带质粒的菌大多有1—2条质粒带,少数含多质粒带。含质粒的菌具有利用五氯酚、四氯酚及三氯酚的能力。

A total of 144 bacterial strains ere isolated fsom the Pesticide (PCP) -polluted soil and water of three geographically different locations situated in North China(Dagu). Central China(Wuhan), and South China (Liuzhou) respectively. Plasmid detection by using agarose gel electrophoresis indicated that 105 strains harboured plasmids, being 72% of the total, while strains isolated from non-polluted samples onlg 3% contained plasmids. The molecular weights of the most plasmids were in the range of 50-100Md. There were one or two plasmids in each of most plasmid harbotring strains, and more than two in a few strains. The plasmid harbouring strains were capable of using pentaehlorophepol, tetrachlorophenol, and trichlorophenol.