肿瘤坏死因子对L_(929)细胞发生细胞毒作用的形态学探讨——光镜及扫描、透射电镜观察

MORPHOLOGY OF CYTOTOXIC EFFECT OF TNF FOR L_(929) CELLS OBSERVATION OF SEM AND TEM

观察肿瘤坏死因子对L_(929)细胞发生细胞毒作用时,靶细胞在间隔时间内的光镜、扫描及透射电镜下的形态学改变,发现光镜下的改变,符合理化因素作用于细胞发生变性、坏死的特点;扫描及透射电镜的动态观察发现,肿瘤坏死因子使靶细胞线粒体增多、肿胀、裂解、溶酶体增多、溶酶体酶释放、细胞器溶解,最后使细胞损伤死亡。故认为肿瘤坏死因子的细胞毒作用不是使细胞膜受到破坏。

Observation of cytotoxic effect of TNF for L_(929) cells, tracing of morphologic change of target cells in intervals time 0, 4, 8, 12, 16 hour, the results are: under microscope, the change accords with denaturatic and necrosic character of general chemical and transmission physical factors effecting on cells.Under scanning electron microscope and transmission electron microscope, the results are: TNF make target cells changes, including: mitochontria swelled; finally cells died.Therefore,cytotoxic effect of TNF is not mainly dissolve of cell membrane