白细胞介素-2对辐射损伤淋巴细胞增殖能力的恢复作用

Effect of IL-2 on Recovery of proliferation Ability of Irradiated T Lymphocytes

本实验将正常人外周血淋巴细胞经^(60)Co γ线照射后与T 细胞激活剂PHA 一起培育,同时加入高度纯化的白细胞介素-2(IL-2),以~3H-TdR 掺入DNA 水平的消长,作为评价IL-2对损伤T 淋巴细胞增殖抑制的恢复作用。结果:经1～40Gy 照射的人外周血淋巴细胞的增殖能力受到抑制,~3H-TdR 掺入DNA 的量随照射剂量的增加而减少。各剂量照射组与不照射组相比,其增殖率仅为27～82%。加IL-2后,各剂量照射组的DNA 均增加,其细胞增殖能力与不加IL-2的照射组相比,1～2.5Gy 照射的淋巴细胞,组间有高度显著性差异:5～10Gy 照射的淋巴细胞,组间差别有显著性;20～40Gy 组间无显著性差异.

~3H-thymidine incorporation assay was used to evaluated the proliferation ability of normal human peripheral blood T Iymphocytes irradiated with or without exogenous IL-2 by ^(60)Co ν-ray at various doses after exposure to 1、2.5、5、10、20 and 40 Gy ν-ray,the DNA synthesis is blocked.It indicated IL-2 has damage effect on the proliferation ability of T cells.~3H-thymidine incorporation rate in cells decreases with increasing dose of irradiation.Incorporation of ~3H-Tdk in irradiated groups in the dose range of 1 to 40 Gy was compared with that in the control group.The incorporation rate of ~3H-TdR in these irradiated groups is 27 to 82% of that in control group.The inhibition of lymphocyte proliferation was partially enhanced by adding IL-2,but the inhibiting effect on proliferation of human peripheral blood lymphocytes exposed to irradiation at more than 10 Gy is not reversible.