参麦液对培养心肌细胞钙矛盾损伤影响的电镜观察

EFFECT OF SHEN MAI INJECTION ON CALCIUM-PARADOX INJURY OF CULTURED MYOCYTET:ELECTRON MICROSCOPIC OBSERVATION

借助透射电镜观察了不同条件下培养乳大鼠心肌细胞的超微结构改变。结果表明正常培养条件下6—7天时,多数心肌细胞含不规则的肌丝或肌原纤维、丰富的线粒体、少量肌浆网和横小管,闰盘亦明显。无钙后给钙培养1小时,多数心肌细胞呈现肌浆网和横小管扩大、线粒体肿胀伴嵴消失,肌节过渡收缩及核膜皱褶等损伤特征。无钙后同时给钙和参麦注射液培养1小时,多数心肌细胞未呈现明显的超微结构病理变化,提示此药能阻抑培养心肌细胞钙矛盾损伤的发生。

The changes of the ultrastructure in cultured myocytes were obs erved with transmission electron microscopy from neonatal rats under different conditions. The results showed that most of the myocardiac cells of 6-7 day culture contained disorganized myofilaments or myofibrils, the great number of mitochondria, and a few sarcoplasmic reticula and the transverse tubules. The intercalated disks were dis tinctly seen. Most of the Myocardiac cells in calcium-free medium followed by recovery in calcium for 1 hour exhibited the characteristic injures including dilated sarcoplasmic reticulum and transveres tubules, and swollen mitochondrias with ther crestae disappearrence, and overcontraction sarcomers, and their ruffled nuclar envelope. However, most of them in calcium-free medium followed by recovery in calcium and given Shen-Mai injection at the same time for 1 hour didn't display Prominent alteration in their ultrastructures. The results suggest this drug can inhibit the occurrence of calcium-paradox injury of the cultured myocardiac cells.