摘要
This paper summarizes the study and application of monoclonal antibodies (McAbs)against haemorrhagic fever with renal syndrome (HFRS) virus in our department over the past sever-al years. The following six points are discussed: (1) establishment and characterization of thehybridoma cell lines secreting McAbs against HFRS virus and hemagglutinin of the virus: (2) theantigenic analysis of HFRS viruses in China by McAbs: (3) purification and application of theMcAbs: (4) purification and characterization of HFRS virus 50K stnactural protein by McAba-affini-ty chromatography and the McAbs possessing different characteristics: (5) detection of HFRS virusantigen in peripheral blood lymphocytes from HFRS patients by the McAb-IFAT; and (6)development of McAb-ELISA indirect sandwich methods, and detection of HFRS virus antigen andIgM, IgG and/or HI antibodies in human and animals. The results of the studies show that theMcAbs can be used for early diagnosis, epidemiological investigation, preparation of vaccine andimmunotherapy of HFRS.
This paper summarizes the study and application of monoclonal antibodies (McAbs) against haemorrhagic fever with renal syndrome (HFRS) virus in our department over the past sever- al years. The following six points are discussed: (1) establishment and characterization of the hybridoma cell lines secreting McAbs against HFRS virus and hemagglutinin of the virus: (2) the antigenic analysis of HFRS viruses in China by McAbs: (3) purification and application of the McAbs: (4) purification and characterization of HFRS virus 50K stnactural protein by McAba-affini- ty chromatography and the McAbs possessing different characteristics: (5) detection of HFRS virus antigen in peripheral blood lymphocytes from HFRS patients by the McAb-IFAT; and (6) development of McAb-ELISA indirect sandwich methods, and detection of HFRS virus antigen and IgM, IgG and/or HI antibodies in human and animals. The results of the studies show that the McAbs can be used for early diagnosis, epidemiological investigation, preparation of vaccine and immunotherapy of HFRS.
