摘要
The lipopolysaccharide (LPS, endotoxin) isolated from Bacteriodes fragilis NCTC 9343 ex-hibted an endogenous heterogencity consisting of R (rough) and SR (semi-rough ) LPS fractionsupon sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The LPS was chemically consti-tuted by D-glucosamine, D-glucose. L-rhamnose, D-galactose. fatty acids and phosphate in amolar ratio of 2: 1: 1: 4: 5. 4.: 2.7 The fatty acids were predominated by 3-hydroxypentadecanoic(3-OH-15: 0), 3-hydroxyhcxadecanoic (3-OH-16: 0), 3-hydroxyheptadecanoic (3-OH-17: 0), 3-hydroxy-15-methyl hexadecanoic (3-OH-15-Me-16: 0) and 13-methyl-letradecanoic acid (13-Mc-14:0). Approximately 1 mol of 2-Keto-3-deoxy-D-mannooctonatc (KDO) was detected after treat-ment of LPS with 48% of hydrofluoric acid (HF), indicating it was present as a phosphorylatedmolecule.
The lipopolysaccharide (LPS, endotoxin) isolated from Bacteriodes fragilis NCTC 9343 ex- hibted an endogenous heterogencity consisting of R (rough) and SR (semi-rough ) LPS fractions upon sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The LPS was chemically consti- tuted by D-glucosamine, D-glucose. L-rhamnose, D-galactose. fatty acids and phosphate in a molar ratio of 2: 1: 1: 4: 5. 4.: 2.7 The fatty acids were predominated by 3-hydroxypentadecanoic(3- OH-15: 0), 3-hydroxyhcxadecanoic (3-OH-16: 0), 3-hydroxyheptadecanoic (3-OH-17: 0), 3- hydroxy-15-methyl hexadecanoic (3-OH-15-Me-16: 0) and 13-methyl-letradecanoic acid (13-Mc-14: 0). Approximately 1 mol of 2-Keto-3-deoxy-D-mannooctonatc (KDO) was detected after treat- ment of LPS with 48% of hydrofluoric acid (HF), indicating it was present as a phosphorylated molecule.
