摘要
A quantitative method using fluorescence spectro-photometer to detect theinterleukin 2 reocptor （IL 2R） on the surface of mononuclear cells in normal humanperipheral blood is described. For expression of IL 2R, the optimum conoentration of PHA andCon A were 100 μg/ml and 10 μg/ml respectively. The PHA induced effect on mononuclear cellswas better than Con A induced one. The peak value of IL 2R cxpression was at the 24th h afterstimulation The optimum dilution of the first antibody （anti-Tac） was 1: 50, while the dilution ofthe second antibody（fluorescein conjugated rabbit anti-mousc IgG） was 1: 32 Their optimumincubation period was 20 and 10 min repectively. The concentration of fluorcscein-conjugatedantibody per 1×10<sup>6</sup> mononuclear cells from the same normal human peripheral blood was5.22±0.45×10<sup>-10</sup> mol, and the coefficient of variation was 8. 6%. The concentration offluorescein-conjugated antibody per 1×10<sup>6</sup>PBMC from five healthy individuals was 4. 8±0. 97×10<sup>-10</sup> mol Therefore this assay system is stable and quite reproducible.
A quantitative method using fluorescence spectro-photometer to detect the interleukin 2 reocptor (IL 2R) on the surface of mononuclear cells in normal human peripheral blood is described. For expression of IL 2R, the optimum conoentration of PHA and Con A were 100 μg/ml and 10 μg/ml respectively. The PHA induced effect on mononuclear cells was better than Con A induced one. The peak value of IL 2R cxpression was at the 24th h after stimulation The optimum dilution of the first antibody (anti-Tac) was 1: 50, while the dilution of the second antibody(fluorescein conjugated rabbit anti-mousc IgG) was 1: 32 Their optimum incubation period was 20 and 10 min repectively. The concentration of fluorcscein-conjugated antibody per 1×10~6 mononuclear cells from the same normal human peripheral blood was 5.22±0.45×10^(-10) mol, and the coefficient of variation was 8. 6%. The concentration of fluorescein-conjugated antibody per 1×10~6PBMC from five healthy individuals was 4. 8±0. 97× 10^(-10) mol Therefore this assay system is stable and quite reproducible.
