摘要
The techniquc of purification of plasma fibroncctin and preparation of its antiserum is re-ported in this paper.Blood plasma was passed through an affinity column of gclatin-Sepharose 4B,fol-lowed by passing throughia column of Scphadex G200.Fibronectin with high purity was obtained.The prepare fibronectin serum.It was found that there were not only specific immune reactions be-tween the anti-human fibronectin serum and hyman fibronectins,but also cross reactions between theanti-serum and the fibronectin from other animals.The serum can be used for the study ofimmunohistochernistry of the fibronectin.
The techniquc of purification of plasma fibroncctin and preparation of its antiserum is re- ported in this paper.Blood plasma was passed through an affinity column of gclatin-Sepharose 4B,fol- lowed by passing throughia column of Scphadex G200.Fibronectin with high purity was obtained. The prepare fibronectin serum.It was found that there were not only specific immune reactions be- tween the anti-human fibronectin serum and hyman fibronectins,but also cross reactions between the anti-serum and the fibronectin from other animals.The serum can be used for the study of immunohistochernistry of the fibronectin.
