摘要
Using plant mini-Ti cosmid pEND4K, the cosmid genomic library of Sesbania rostratawas constructed. Sizes of plant DNA inserts in clones were 25- 33 kb. The restrictionmapping showed that different recombinant involved various types of plant DNA insert. 4clones containing leghemoglobin gene sequence of S. rostrata were obtained by in situ hy-bridization of colonies. The cloning of leghemoglobin gene sequence has been confirmedby plasmid DNA dot hybridization and Southern blot hybridization.
Using plant mini-Ti cosmid pEND4K, the cosmid genomic library of Sesbania rostrata
was constructed. Sizes of plant DNA inserts in clones were 25- 33 kb. The restriction
mapping showed that different recombinant involved various types of plant DNA insert. 4
clones containing leghemoglobin gene sequence of S. rostrata were obtained by in situ hy-
bridization of colonies. The cloning of leghemoglobin gene sequence has been confirmed
by plasmid DNA dot hybridization and Southern blot hybridization.
基金
Project supported by the National Natural Science Foundation of China.
