摘要
采用 SPA 协同凝集试验(CoA 法)直接从抗生素相关性腹泻(antibioticassociated diarrhea,AAD)病人粪便中检测难辨菌的毒素,以达到快速诊断的目的。与厌氢培养法、细胞毒素试验、CIE、ELISA 作比较,CoA 法简便、快速、显示了较高的特异性和敏感性,并且不需要特殊设备,易于推广应用,是一种快速早期诊断抗生素相关性腹泻的有效方法。
In order to diagnose the antibiotic associated diarrhea(AAD)rapidly we develo- ped a Staphylococcal Protein A-Coagglutination Assay(CoA)for detection of Clostri- dium difficile toxins in fecal specimens from patients with AAD.The results revealed that the specificity and sensitivity of CoA to Clostridium difficile toxins were higher than those of anaerobe culture,cell toxin test,counterimmunoelectrophoresis(CIE), and Enzyme-linked immunosorbent assay(ELISA).CoA is thought to be a technique without any special apparatus for rapid and early diagnosis of AAD.
出处
《东南大学学报(医学版)》
CAS
1989年第3期1-6,共6页
Journal of Southeast University(Medical Science Edition)
关键词
难辨梭菌
SPA协同凝集试验
腹泻
诊断
Clostridium difficile
Staphylococcal Protein A-Coagglutination
diarrhea
diagnosis