人癌基因c—sis 的RFLP_s在肝癌和正常组织中的分布

THE DISTRIBUTION OF RESTRICTION FRAGMENT LENGTH POLYMO RPHISM OF HUMAN C-SIS ONCOGENE IN HEPATOMA AND NORMAL TISSUES

作者用人 c-sis 基因1.7kb 探针(BamHⅠ—BamHⅠ)研究了 c-sis 基因的 RFLps在肝癌和正常组织中的分布。并首次报道 c-sis 基因对 HnidⅢ、EcoRⅠ、SacⅠ、PstⅠ、PvuⅡ和 MspⅠ六种限制酶有多态性,其中用 EcoRⅠ、PatⅠ、PvuⅡ、和 MspⅠ四种限制酶、发现的 c-sis 基因 RFLPs 在肝癌和正常组织中无明显差别。本文还对 c-sis 基因甲基化问题进行了讨论,并表明 c-sis 基因的 GGCC 顺序甲基化程度较低且无组织特异性:

The restriction fragment length polymorphisms(RFLPs)of human c-sis oncogene for Hind Ⅲ,EcoR I,Sac Ⅰ,Pst Ⅰ,Msp Ⅰ,Pvu Ⅱ,and Hae Ⅲ,and their distribu- tion in human hepatoma and normal tissues were studied with 1.7kb c-sis pro(?)e(BamH Ⅰ-BamH Ⅰ).The RFLPs of human c-sis oncogene for all the enzymes tested but Hae Ⅲ were reported.No difference in the distribution of the RFLPs of human c-sis gene for Pst Ⅰ,Pvu Ⅱ,EcoR Ⅰand Msp Ⅰ was observed between human hepatoma and nor- mal tissues.Interestingly,Hind Ⅲ detects only 4.2kb and 2.4kb invariable fragments in human hepatoma,in addition to these two fragments,6.3kb fragment was observed in all the blood sambles.The 6.3kb fragment was also found in uterine muscles and myometrium.According to the above results,three possible explanations are suggested. Hypomethylation of GGCC seguence in human c-sis gene was exhibited in plenty of tissues.