一种改良的艰难梭菌分离方法

A MODIFIED METHOD FOR THE ISOLATION OF CLOSTRIDIUM DIFFICILE

艰难梭菌是人类抗生素相关性结肠炎的主要病原体。该菌分离困难。本文介绍了一种改良的乙醇处理选择培养艰难梭菌的方法,从地鼠抗生素相关性结肠炎模型中分离艰难梭菌阳性率达93.9%,优于 CCFA 选择培养基(阳性率36.4%)。此方法分离阳性率高,成本低,材料易得,简便易行,值得推广应用。

Clcstridium difficile is the main pathogenic bacterium of antibiotic-associated col- itis(AAC).It is difficult to isolate Clostridium difficile from specimens,A modified method for its isolation with alcohol treatment at the concentration of 70% for 10 mi- nutes was introduced,As high as 93.9%(31/33)of positive rate was obtained for isola- tion of Clostridium difficile from the models of AAC in hamsters with the modified metrod compared with 36.4%(12/33)by the selective medium of cefoxitin,cycloserine, fructose,and agar.