摘要
本试验收集了不同地区的谷子品种120个,采用聚丙烯酰胺凝胶电泳法,在芽期进行了酯酶同工酶分析。试验结果表明,(1)谷子芽期的酯酶同工酶是遗传稳定的性状。谷子芽期的酯酶同工酶共出现了19条酶带,其中 E_()区为主酶带区,E_1(1-3)、E_2(5)、E_2(6)、E_2(7),E_4(14),E_5(15)酶带是谷子芽期酯酶同工酶的基本酶带。(2)从被测的120个品种中,获得了31种酯酶同工酶酶谱类型。分析表明,谷子芽期的酯酶同工酶具有较为明显的种(或品种)的专一性,可以作为谷子分类的生化指标之一。(3)从地理分布的分析表明,内蒙高原区和西北高原区酯酶同工酶酶谱类型多,品种变异丰富,进化程度高,可能是栽培谷子的起源地区。
One hundred and twenty sprouting foxtail millet varieties collected from various locations in China were analyzed by polyacrylamide gel electrophoresis. The results are as follows; 1 The isozymes of esterases in foxtail millet were stationary in genetic characters.Of the 19enzyme bands found in testing the sprouting foxtail millet, E_4 was the main enzyme zone,the bands E_1(1-3),E_2(5),E_2(6),E_2(7), E_4(14),and E_5(15)were the basic bands. 2 Thirty one zymogram types of esterase-isozyme patterns were obtained. The isozymes of esterases in sprouting,foxtail millet werse with obvious speclf -icity of the varieties,which can be used as biochemical indexes for researches on classification of foxtail millet. 3 The types of the isozymes of esterases in Inner Mongolian Plateau and Northwest Plateau were more complex,varieties were very abundant with high degrees of evolution,indicating that these regions are possibly the original re- gions of foxtail millet varieties.
关键词
谷子
凝胶电泳法
酯酶同工酶
Foxtail millet
Polyacrylamide gel electrophoresis method
Isozymes of esterases
