15种化合物在果蝇非整倍体检测系统中的诱变效应

MUTAGENETIC EFFECT OF 15 COMPOUNDS IN DROSOPHILA ANEUPLOIDY DETECTING SYSTEM

本试验所用黑腹果蝇非整倍体测试品系为yB/Yy+和yw/yw,两者都是在我们用甲基磺酸乙酯诱发的突变型y和yB的基础上通过遗传杂交培育而成的。为了确定该测试系统的敏感度,用15种化合物进行了试验。其中6种已作过致癌性的明确分类(全为致癌阳性物)。这6种里有5种在本试验中确定为诱发染色体丢失的阳性物,敏感度为83％;有3种确定为诱发染色体不分离的阳性物,敏感度为50％。

Both yellow(y/Y) and yellow-Bar(yB/Y) mutants of Drosophila melanogaster had been occasionally found by the sex-linked recessive lethal test for determing genetic safety of medical silicium rubber when Oregon K males are treated with ethyl methanesulfonate. The yw(yw/yw and yw/Y)were obtained by using y/Y(?) mated with w/w(?) · The yB(yB/yB and yB/Yy+)were obtained by using yB/yB(?) mated to R(1)(?) (yB/Yy+). The females yw and males yB/Yy+ are used in ane-uploidy testing. Total 15 chemicals were detected in our lab. Tested chemicals include: colcemid(1), colchicine(2), caffeine(3), actinomycine-D(4), MNNG(5), 5-flurodcoxyuridine(6), proflavine (7), cyclophosphamide (8), mitomycin-C (9), meprobamate (10), sorbitor (11), urethane (12), methyl methanesulfonate (13), sodium fluoride(l4), 5-bromodeoxyuridine(l5). Normal test described by Margolin et al (1983) anb Kastenbaum-Bowman test are used in examine data for statistical significance. The compounds which significant difference at the 5% level between the treated and control frequencies of chromosome gain in treated(?) include(1, 2, 5, 7, 9, 14), and (4) intreated females. The compounds which significant difference at the 5% level of chromosome loss in treated males include (3, 4, 6, 7, 8, 9, 11, 12, 13, 14, 15), and(l,5) in treated females. Of 15 compounds tested for non-disjunction and loss, only six (4, 5, 8, 9, 12,13) have definitive carcin-ogenesis classifications (allpositive). Five(4, 8, 9, 12, 13) of these were significant difference for loss, three (14, 5, 9)of these were significant difference for non-disjunction. Sensitivity is 83% and 50%, respectively.