测定棉籽中游离棉酚的改良方法

A MODIFIED METHOD FOR DETERMING THE FREE GOSSYPOL IN COTTON SEED

本文对间苯三酚法测定游离棉酚进行了深入研究。改变了原方法中的溶剂抽提系统,显色剂的配方作了改进。在此基础上,研究了显色的最佳条件。便得本文提出的间苯三酚改良法,在准确度、灵敏度、线性范围加大等方面,优于原间苯三酚法。两人一日至少可分析30～40个样品。

A new phloroglucinol method is proposed to determine free gossypol in cotton seed.This new method has changed the extracting agent of original phloroglucinol method, developing agent preparation is also improved. Under the above conditions, this article has investigated the effects of PH, developing agent concentration, developing time and temperature etc.The sensitivity of this new phloroglucinol method is highly improved: color stability, accuracy and precision are also better; linear concentration range is enlarged, the rapidity of analysis is highly improved. Therefore, this new method is superior to the original phloroglucinol method. 30-40 samples can be finished by two workers each day.