超氧化物歧化酶和U74389G对新生鼠高氧肺损伤的治疗作用

The therapeutical effects of SOD and U74389G on hyperoxic lung injury in newborn rats

目的:探讨抗氧化剂治疗高氧肺损伤的疗效及机制。方法:将新生SD大鼠随机分为对照组、高氧组、SOD组和U74389G组,每组14只。复制高氧肺损伤模型,用SOD和U74389G进行治疗。黄嘌呤氧化酶法测定SOD活性,硫代巴比妥酸法测定MDA含量,TUNEL法检测细胞凋亡,观察肺病理变化以及放射状肺泡计数(RAC)。结果:高氧组的肺系数、MDA含量和凋亡指数显著高于对照组,RAC明显减少(均P<0.01);U74389G组及SOD组与高氧组相比,肺系数下降、MDA含量减少、SOD活性增高、凋亡指数下降,RAC升高(P<0.01,P<0.05)。高氧组肺组织严重水肿,中性粒细胞浸润,SOD组肺组织病理改变明显减轻,U74389G较高氧组有所减轻。SOD组在缓解肺系数、降低SOD活性及抑制凋亡方面优于U74389G组(P<0.01,P<0.05);U74389G组在抑制MDA含量方面优于SOD组(P<0.01),而两组间RAC差异无显著性。结论:抗氧化剂U74389G和SOD能减轻高氧肺损伤。

Objective： To explore the mechanism and therapeutical effect of anti-oxidation on hyperoxic lung injury. Methods ： Newborn Sprague Dawley rats were randomly divided into 4 groups： control group, hyperoxia-exposed control one, SOD one and U74389G one. There were fourteen rats in every group. Copy the model of hyperoxic lung injury. SOD or U74389G was given to the treatment groups. Contents of MDA and SOD activity were measured, cell apoptosis was detected, the pathological changes and RAC were observed under light microscope. Results ： The hyperoxia-exposed control group was significantly higher than that of the control groups in coefficient of lung, contents of MDA and apoptosis index. PAC was significantly decreased （P〈0.01）.The U74389G group and SOD group were better than hyperoxia-exposed control group in coefficient of lung, SOD activity, contents of MDA, RAC and apeptosis index, （P〈0.01,P〈0.05）. Hyperxia- exposed control groups＇ lung tissues were severe edema, neutrophil infiltration, SOD group were signifi- cantly improved, but U74389G group improved a little. SOD group was significantly better than U74389G group in coefficient of lung, SOD activity and apoptosis index, （P〈0.01, P〈0.05）. U74389G group was significantly better than SOD group in contents of MDA,（P〈0.01）, and there was no significant difference between U74389G group and SOD group in RAC. Conclusion： Antioxidant U74389G and SOD can relieve hyperoxic lung injury.