人胎肝细胞低温保存的临床基础研究

Studies in cryopreservation of fetal liver cells in man

近年来,随着低温医学技术和实验血液学研究的进展,业已证明低温保存的自体骨髓治疗白血病及淋巴瘤等患者取得较好疗效。由于胎肝细胞不仅能使造血重建,而且可减少或避免移植物抗宿主病(GVHD)的发生,因此人们对胎肝细胞移植寄于很大希望。为了得到足够的胎肝细胞来源,很多学者进行了胎肝细胞低温保存的研究。

Thirty-seven specimens of human fetal livr cells, obtained during spontaneous bortion and stored in liquid nitrogen for 1 to 793 days, were evaluated for viability using he ranulocyte-macrophage (GM-CFU-C) assay and the dye exclusion test. In the postthawing fetal iver suspension, the rates of nuclear cell recovery and trypan blue exclusion were 97.97±0.68% and 75.68±17.29% respectively, with a mean GM-CFU-C yield of 218.63±139.50/2×10~5 ucleated cells.Eleven patients with aplastic anemia, acute leukemia, and liver failure have een given infusions of thus cryopreserved fetal liver cells. These patients were benefited rom his highdose cytoreductive therapy with cryopreserved fetal liver cells. Two children with cute leukemia showed sucessful engraftment and the graft was proved by means of genetic arkers s the donor's karyotypes.The data presented here indicate that human fetal liver cells can be reserved in -196℃ for two years without significant loss of viability, and cryopreserved etal iver cells can be used with success in clinical practice without inducing harmful side ffects.