一种测定CuZn-SOD总量的新方法——化学发光免疫分析法

A new method for estimating toral CuZn-SOD ——Chemiluminescent immunoassay

通过探索,用ABEI发光剂标记人CuZn-SOD,液相竞争结合测定样品中CuZn-SOD含量。本方法试剂稳定,结果重复性好,批内CV=2.6～6.0％,批间CV=6.9～7.9％,回收率97.7～106.1％,可测范围为1～500ng,适合测定血清及体液中微量CuZn-SOD。本化学发光免疫分析为国内外尚未报道的方法。

The human CuZn-SOD labelled with 6[N-(4-aminobutyl)-N-ethyl]-isoluminol (ABEl) has een used for estimating total CuZn-SOD, and then a new method of chemiluminescent immunoassay as established. Inter-assay coefficients of variation were 2.6—6.0％ and 6.9—7.9％ espectively. The recoveries of human CuZn-SOD added in sample were 97.7—106.1％. The assay as roved to be reliable, accurate and the limit of determination was 1—500 ng. The method has een successfully applied in the determination of total CuZn-SOD content in serum or xtracellular fluids.