狂犬病毒糖蛋白抗原决定簇的筛选及其氨基酸序列分析

Analysis of amino acid sequences of antigenic domains of the rabies virus glycoprotein

狂犬病是一种人畜共患的烈性传染病,在世界范围内广泛传播。狂犬病病毒的结构蛋白主要由糖蛋白(G)、核蛋白(N)、磷酸化蛋白(NS)、基质蛋白(M)所组成。糖蛋白分子量为80,000,是狂犬病病毒的一种主要的抗原成分,它诱导了病毒中和抗体的产生,与抗感染免疫能力有密切关系。近来,人们对糖蛋白的抗原结构进行了研究,根据用中和性的单克隆抗体对糖蛋白的反应性的模型,

To locate epitopes on glycoprotein antigen of rabies virus, the glycoprotein was urified by SDS-PAGE. The purified glycoprotein was digested at glutamic acid residues by rotease V8, transferred to nitrocellulose. The McAbs directed against the rabies virus lycoprotein have been used to delineate the site on the glycoprotein. One McAb-positive ragment of glycoprotein (G-V-6) was Selected by immunostaining. The N-terminal sequence of the urified fragment was determined by direct amino acid sequence analysis. The results indicat hat this method leads to the elucidation and rnapping of the antigenic determinant.