纳米羟基磷灰石粉体的生物活性的研究

Investigations of Biological Activity of Nanohydroxyapatite Ceramic Powder

采用溶胶-凝胶法制备羟基磷灰石陶瓷材料。通过控制工艺参数:pH>9,Ca/P=1.67,烧结温度为1100℃及烧结时间2h,合成出纯度较高纳米粒径的羟基磷灰石粉体。通过羟基磷灰石对不同氨基酸的吸附强度,选取显著吸附的氨基酸进行紫外分光光度法、X射线衍射(XRD)物相分析和X射线衍射谱(XDS)分析,研究羟基磷灰石粉体的结构和吸附氨基酸的生物活性。结果表明:羟基磷灰石能吸附氨基酸,吸附位点是PO43-基,而不是Ca2+。提出羟基磷灰石吸附具有不同电位的氨基酸的作用机理。

The nanohydroxyapatite ceramic powder was synthesised by using sol-gel method. The work parameters were controlled as pH〉9, Ca/P= 1.67, the sintered temperature and time were controlled at 1100℃ and 2 hours, respectively. A nanometer powder of hydroxyapatite with high purity were obtained. Its structure and biological activity absorbing the amino acid molecules were studied by ultraviolet absorption spectrum, X-Ray Diffraction （XRD）structure analysis, infrared spectrum and X-ray Diffraction Spectrum （XDS） of constitute analysis. The results showed that the nano- hydroxyapatite can absorb these amino acid molecules, of which acted positions on the hydroxyapatite is the radical PO4a-, instead of Ca^2＋. The mechanism of absorbing of the hydroxyapatite with amino acid molecules with different electric potentials was proposed.