摘要
对36个命名为秀珍菇的菌株进行ITS特异性扩增,结果显示菌株间没有多态性.根据ITS-RFLP及ITS序列,将36个菌株分为两类,第1类菌株包括Pl.g 0003、Pl.g 0006、Pl.g 0009、Pl.g 0016和Pl.g 0026,是糙皮侧耳,不是秀珍菇;余下31个菌株归入第2类,是凤尾菇,也不是秀珍菇.ITS-RFLP可应用于商业秀珍菇种性的鉴定.
Internal transcribed spacer (ITS) was specially amplified for thirty-six Pleurotus geesteranus strains. Results showed that there was no length polymorphism among strains. Thirty-six P. geesteranus strains were divided into two classes based on ITS-RFLP and ITS sequences. The first class including P1. g 0003, P1. g 0006, P1. g 0009, P1. g 0016 and P1. g 0026 belonged to Pleurotus os- treatu. Other thirty-one strains belonged to the second class. Strains of the second class are Pleurotus pulmonarius but not P. geesteranus. ITS-RFLP could be applied for identification of "P. geesteranus" species.
出处
《福建农林大学学报(自然科学版)》
CSCD
北大核心
2009年第2期186-191,共6页
Journal of Fujian Agriculture and Forestry University:Natural Science Edition
基金
福建省科技厅资助项目(2006S1001)
关键词
秀珍菇
同名异物
ITS-RFLP
Pleurotus geesteranus
different species with same name
ITS-RFLP