无机氟对大鼠生物膜脂质流动性的影响

Effect of fluoride on the lipid fluidity of biomembranes of rats

以ＡＮＳ，ＤＰＨ为荧光探剂，用荧光偏振技术观察氟对生物膜流动性的影响。体外实验，终浓度１～１２８ｍｍｏｌ／ＬＮａＦ对大鼠脑突触体上ＤＰＨ的荧光偏振度（Ｐ值）无明显影响（Ｐ＞０．０５）；而ＡＮＳ的Ｐ值增大，即膜表层流动性降低，呈剂量－效应关系。体内以每日２０ｍｇ／ｋｇＮａＦ腹腔注射染毒５０天，观察大鼠脑突触体膜，肝细胞膜和红细胞膜的平均流动性变化，发现３种膜中ＤＰＨ的Ｐ值均显著升高（Ｐ＜０．０１）。提示氟的直接作用和通过影响体内代谢导致生物膜流动性降低。

ffect of fluoride on the lipid fluidity of some biomembranes of rats was determined by fluorescence polarization technique using DPH and ANS as fluorescence probes in vitro and in vivo in order to further understand the mechanism of cellular effect and membrane toxicity of fluoride. When exposure doses of fluoride from 1 to 128 mmol/L, the fluorescence polarization of ANS in synaptic membrane was increased significantly with obvious sode effect relationship (r=0.85, P<0.05), but the P of DPH was not altered in vitro. In vivo, sodium fluoride at 20mg/kg·day was injected intraperitioneally for 50days, the results showed that the fluorescence polarization of DPH in synaptic membrane, liver cell and erythrocyte membranes were significantly elevated. Considering the chemical properties of fluoride and the results, it is likely that effect of fluoride on the lipid fluidity of biomembranes probably result from either the direct interaction with biomembrane surface structures, or interference with lipid metabolism of biomembrane.\ \