不同时间干细胞移植对于脊髓损伤大鼠的作用

Stem Cell Transplantation at Different Time Points after Spinal Cord Injury for Behavioral Recovery in Rats

目的:比较脊髓损伤后不同时间神经干细胞移植对于大鼠功能恢复的治疗效果方法:33只雌性SD大鼠用于本次实验,4只进行椎板切除作为假手术对照组,其余进行胸9水平脊髓半切。脊髓半切大鼠分为5组,分别在脊髓损伤后1、3、7、14和30d进行神经干细胞移植。行为学测试用于评价行为恢复。结果:在3、7、14d接受干细胞移植的大鼠有较高的BBB测试的分值并在网格测试中较少摔倒。脊髓损伤后第7d干细胞移植的大鼠行为功能恢复的最好。结论:不同时间移植神经干细胞的行为恢复效果不同。脊髓损伤后从第3天至第14天进行神经干细胞移植能获得治疗效果。损伤后1周移植的效果最好。

Objective：To compare therapeutic effect of neural stem cells（NSCs） transplantation at different time points on functional recovery after spinal cord injury（SCI）.Methods：Thirty three female Sprague-Dawley rats（250300g） were subjected to the experiment.Except sham-operation rats,all the rats received spinal cord hemisection on the right side at T9 level.Hemisected rats were randomly assigned to spinal cord injury group and 5 NSCs groups.Rats in NSCs groups received NSCs transplantation on the 1st,3rd,7th,14th and 30th days after SCI.The behavioral recovery effect was evaluated through behavioral test.Results：Rats received transplantation on the 3rd,7th and 14th days after SCI got higher scores in BBB test and fewer footfalls in grid test.The best result in behavioral tests was among the group that received transplantation 7 days after SCI.Conclusion：NSCs transplantation at different time points can result in different behavioral recovery.The curative effect can be observed from the 3rd day to the 14th day after SCI,but the best effect is obtained on the 7th day.