针刺对脑出血大鼠继发性神经损伤保护机制的研究

Nerve protection mechanism of acupuncture to the secondary injury in the rat of intercerebral hemorrhage

目的 探讨针刺对脑出血大鼠继发性神经损伤保护作用的机制。方法选取健康雄性Wistar大鼠160只，先随机分为：模型对照组（简称模型组），模型＋针刺组（简称针刺组），模型＋脑复康组（简称脑复康组），每组50只大鼠，每组又分为术后6h、1d,2d、3d,7d5个时相点，每个时相点10只大鼠，制备脑出血模型；另设10只正常大鼠作为空白对照组（简称正常组）。针刺组针刺患侧百会透曲鬓穴。观察各组大鼠血肿周围脑组织神经生长因子（NGF）的表达。结果针刺组大鼠血肿周围脑组织NGF的表达随不同时相点的变化而逐渐增加，至7d时达高峰，与模型组比较差异有统计学意义（P〈0．01）。结论针刺能够促进脑出血大鼠血肿周围脑组织NGF的表达。

Objective Expwre to the mechanism of the function of nerve protection to the secondary injury in the rat of intercerebral hemorrhage by acupuncture. Methods 160 healthy Wistar male rats were selected. The rats were assigned into 3 group randomly： model group, pinprick set, piracetam group, containing 50 rats in each. Each group were again divided into five time phase point of 6 hours, 1 day,2days ,3days ,7days. containing 10 rats in each. All rats were made to be the intercerebral hemorrhage model. Another 10 rats were assigned as the control. The pinprick group were acupunctured on the ＂Baihui＂ penetrating＂ Qubin＂. Results In the pinprick group, the expression of NGF of rat＇s brain tissue surrounding the hematoma was increased gradually and to the peak in 7days with the increasing of different time phase point . In the same time phase point, compare with the model group, the pinprick group＇s expression of NGF was higher obviously. Both were relatively showed the significant difference （ P 〈 0.01 ） and had statistical significarde. Conclusion The therapy of acupuncture can promote the expression of NGF of the rat＇s brain tissue surrounding the hematoma.