摘要
本文报道以荧光原位杂交技术(FISH)及抗着丝粒抗体间接免疫荧光染色(CREST染色)两种技术检测了化合物甲基丙烯酸环氧丙酯(GMA)诱发微核的着丝粒信号比例,从而判断GMA的非整倍体诱发效应。结果表明,GMA诱发的微核约40.9-575%为FISH阳性,说明其具有一定的非整倍体毒性,但CREST阳性仅为170-241%,说明其对着丝粒蛋白有较强的损害作用。同时,染色体中期相分析表明,GMA的染色体断裂作用较强。
In this research Fluorescence in situ hybridization (FISH) and antikinetochore antibodies (CREST Syndrome serum) were used in determining the effect of inducing aneuploid of GMA. The two techniques mainly detected the proportion of centric signals in micronuclei induced by GMA. The results showed that FISH positive ratio of GMA was 40.9-51.5%, but for CREST staining it was only 17.0-24.1%. It indicated that GMA was a suspected spindle poison and could damage the centric protein. The metaphase analysis demonstrated that GMA had significantly clastogenic effect, which mostly happened in centromere region.
出处
《癌变.畸变.突变》
CAS
CSCD
1998年第2期65-68,共4页
Carcinogenesis,Teratogenesis & Mutagenesis
关键词
GMA
荧光原位杂交
抗着丝粒抗体
非整倍体毒性
micronuclei
glycidyl methacrylate
Fluorescence in situhybridization
antikinetochore antibodies
aneuploid effect