荧光原位杂交和CREST染色对甲基丙烯酸环氧丙酯的非整倍体诱发效应的研究

STUDY OF THE ANEUPLOID EFFECT OF GLYCIDYL METHACRYLATE (GMA) USING FLUORESCENCE IN SITU HYBRIDIZATION AND CREST STAINING

本文报道以荧光原位杂交技术（ＦＩＳＨ）及抗着丝粒抗体间接免疫荧光染色（ＣＲＥＳＴ染色）两种技术检测了化合物甲基丙烯酸环氧丙酯（ＧＭＡ）诱发微核的着丝粒信号比例，从而判断ＧＭＡ的非整倍体诱发效应。结果表明，ＧＭＡ诱发的微核约４０．９－５７５％为ＦＩＳＨ阳性，说明其具有一定的非整倍体毒性，但ＣＲＥＳＴ阳性仅为１７０－２４１％，说明其对着丝粒蛋白有较强的损害作用。同时，染色体中期相分析表明，ＧＭＡ的染色体断裂作用较强。

In this research Fluorescence in situ hybridization (FISH) and antikinetochore antibodies (CREST Syndrome serum) were used in determining the effect of inducing aneuploid of GMA. The two techniques mainly detected the proportion of centric signals in micronuclei induced by GMA. The results showed that FISH positive ratio of GMA was 40.9-51.5%, but for CREST staining it was only 17.0-24.1%. It indicated that GMA was a suspected spindle poison and could damage the centric protein. The metaphase analysis demonstrated that GMA had significantly clastogenic effect, which mostly happened in centromere region.