胆汁酸的遗传毒性和对细胞间隙连接通讯(GJIC)抑制作用的研究

STUDIES ON THE GENOTOXICITY AND INHIBITORY EFFECT ON GAP JUNCTION INTERCELLULAR COMMUNICATION OF BILE ACID

本文应用Ａｍｅｓ试验，ＣＨＬ细胞体外染色体畸变试验检测了脱氧胆酸和石胆酸的遗传毒性；还观察了脱氧胆酸和石胆酸对ＮＩＨ／３Ｔ３细胞的恶性转化作用。并应用划痕标记染料示踪技术（ＳＬＤＴ）观察了胆汁酸对细胞间隙连接通讯（ＧＪＩＣ）的抑制作用。结果表明，脱氧胆酸和石胆酸无诱发基因突变和染色体畸变作用，也无诱发培养细胞的恶性转化作用。因此本实验未证明脱氧胆酸和石胆酸是遗传毒性致癌物。然而，脱氧胆酸和石胆酸对ＮＩＨ／３Ｔ３细胞ＧＪＩＣ均有明显抑制作用。

The genotoxicities of Deoxycholic acid and Lithocholtic acid were tested by Ames test and chromosome aberration test in vitro. The malignent transformation effect of Deoxycholic acid and Lithocholic acid on NIH/3T3 cells was observed, The inhibitory effect of Deoxycholic acid and Lithocholic acid on gap junction intercellular communication (GJIC) in NIH/3T3 cells was investigated with Scrap Loading/Dye Transfer test (SLDT). The results indicated that neither Deoxycholic acid nor Lithocholic acid has gene mutation, chromosome aberration and cell malignant transformation in vitro, Therefore, it did not demonstrate that bile acid is a genotoxic carcinogen. But result showed that both Deoxycholic acid and Lithocholic acid could inhibit NIH/3T3 cells GJIC obviously. It means that bile acid may play a role as promoter in carcinogenesis.