摘要
本研究采用微团培养观察了V2O5对大鼠胚胎中脑细胞增殖和分化的影响,旨在为进一步揭示其发育毒作用机理提供依据。结果显示,V2O5对体外中脑细胞的增殖和分化均有抑制作用,并有明显的剂量-反应关系。V2O5抑制中脑增殖和分化的IC50分别为91和68μmol/l。体内/体外结合试验显示,V2O5在20mg/kg时对细胞分化有明显的抑制作用,而在50mg/kg时才对细胞增殖出现抑制作用,按照Renault的分类标准。
Vanadium is a proven animal teratogen which can induce dilation of latermal ventricles and skeletal abnormal in rat, although the mechanism of its developmental toxicity is unknown. In this paper, the micromass rat embryo midbrain cell culture was used in a affort to determine potential mechanisms by which Vanadium Pentoxide (V 2O 5) may exert its teratogenic effect. In vitro test, a dose dependent inhibition of differentiation and proliferation of midbrain cells were observed. The 50%-inhibitory concentration (IC 50 ) for cell differntiation and cell survial or proliferation 50% the control value(IC 50 ) are 6.8 and 9.1μmol/L, respcectively. In vivo/in vitro test, V 2O 5 could inhibit cell differentiation at 2.0 mg/kg body weight, but inhibit cell surival or proliferation at 5.0mg/kg. According to class standard of Renault, el al(1989), V 2O 5 may be a specific inhibitor of embryonic midbrain cell.
出处
《癌变.畸变.突变》
CAS
CSCD
1998年第2期81-84,共4页
Carcinogenesis,Teratogenesis & Mutagenesis
关键词
微团培养
胚胎
脑细胞增殖
五氧化二钒
发育毒性
Micromass Culture
Embryo midbrain cell
Rat
Vandium Pentoxide
Developmental toxicity