五氧化二钒对大鼠胚胎中脑细胞增殖和分化的影响

EFFECTS OF VANADIUM PENTOXIDE ON RAT EMBRYO MIDBRAIN CELL PROLIFERATION AND DIFFERENTIATION

本研究采用微团培养观察了Ｖ２Ｏ５对大鼠胚胎中脑细胞增殖和分化的影响，旨在为进一步揭示其发育毒作用机理提供依据。结果显示，Ｖ２Ｏ５对体外中脑细胞的增殖和分化均有抑制作用，并有明显的剂量－反应关系。Ｖ２Ｏ５抑制中脑增殖和分化的ＩＣ５０分别为９１和６８μｍｏｌ／ｌ。体内／体外结合试验显示，Ｖ２Ｏ５在２０ｍｇ／ｋｇ时对细胞分化有明显的抑制作用，而在５０ｍｇ／ｋｇ时才对细胞增殖出现抑制作用，按照Ｒｅｎａｕｌｔ的分类标准。

Vanadium is a proven animal teratogen which can induce dilation of latermal ventricles and skeletal abnormal in rat, although the mechanism of its developmental toxicity is unknown. In this paper, the micromass rat embryo midbrain cell culture was used in a affort to determine potential mechanisms by which Vanadium Pentoxide (V 2O 5) may exert its teratogenic effect. In vitro test, a dose dependent inhibition of differentiation and proliferation of midbrain cells were observed. The 50%-inhibitory concentration (IC 50 ) for cell differntiation and cell survial or proliferation 50% the control value(IC 50 ) are 6.8 and 9.1μmol/L, respcectively. In vivo/in vitro test, V 2O 5 could inhibit cell differentiation at 2.0 mg/kg body weight, but inhibit cell surival or proliferation at 5.0mg/kg. According to class standard of Renault, el al(1989), V 2O 5 may be a specific inhibitor of embryonic midbrain cell.