凋亡抑制蛋白c—FLIP在扁平苔癣患者外周血淋巴细胞和皮损中的表达

Expression of cellular FLICE-inhibitory protein in peripheral blood and lesion of patients with lichen planus

目的探讨凋亡抑制蛋白c—FLIP（细胞型Fas相关死亡区域蛋白样IL-1β转换酶抑制蛋白）在扁平苔癣患者外周血和皮损中的表达和分布情况。方法采用流式细胞仪检测30例扁平苔癣患者和20例正常人对照组外周血T细胞内c—FLIP表达阳性率，采用免疫组化方法检测c—FLIP在其皮损中的表达。结果扁平苔癣患者外周血T细胞c—FLIP表达（6．32％±1．17％）明显高于正常人对照组（2．28％±0．54％），P〈0．05。而两组在外周血B细胞内的表达分别为0．78％4±0．16％和0．69％±0．18％，差异无统计学意义（P〉0．05）。c—FLIP蛋白在扁平苔癣患者皮损中的表达（89．73％±5．24％）明显高于正常人对照组（121．58％±7．93％），P〈0．01。结论凋亡抑制蛋白c—FLIP在扁平苔癣患者的外周血T细胞和皮损内明显高表达，可能参与扁平苔癣患者T细胞的增殖。

Objective To investigate the expression and distribution of cellular FLICE （ Fas-associated death domain-like interleukin-1 B-converting enzyme-like）-inhibitory protein （c-FLIP） in peripheral blood and lesions of lichen planus patients. Methods Peripheral blood and skin samples were obtained from 30 patients with lichen planus and 20 normal controls. Flow cytometry was used to detect intracellular c-FLIP in peripheral T and B lymphocytes, and immunohistochemistry was used to examine the expression of c-FLIP in lesional tissue. Results Based on the positivity rate of c-FLIP, there was a significant increase in T lymphocytes in lichen planus compared with normal controls（6.32% ± 1.17% vs 2.28% ±0.54% , P 〈 0. 05） , while no significant difference was found in B lymphocytes between two groups （0.78% ±0.16% vs 0.69% ±0. 18% , P 〉 0. 05 ）. The expression intensity of c-FLIP in keratinocytes was also higher in lichen planusthanthatinnormalcontrols （ 89.73% ± 5.24% vs121.58% ± 7.93% ,P 〈 0.01 ）. Conclusion c-FLIP is highly expressed in lesions and peripheral T lymphocytes of patients with lichen planus, which suggests the possible involvement of c-FLIP in the proliferation of T lymphocytes in lichen planus.