抗巨噬细胞活化趋化因子-1抗体对脑缺血-再灌注大鼠保护作用的实验研究

Experimental study on protective effect of antimacrophage activating chemokine1 antibody on cerebral ischemiareperfusion in rats

目的：探讨抗巨噬细胞活化趋化因子１（Ｍａｃ１）抗体保护神经元缺血性损伤的作用机制。方法：２４只Ｗｉｓｔａｒ大鼠随机分为４组：正常对照组（４只）、伪手术组（４只）、脑缺血再灌注损伤组（脑缺血组，８只）、脑缺血再灌注损伤加Ｍａｃ１抗体实验组（脑缺血加抗体组，８只）。分离培养鼠脑毛细血管内皮细胞（ＣＣＥＣ）和多形核白细胞（ＰＭＮ），利用微管吸吮技术，观察ＰＭＮ与ＣＣＥＣ间粘附力学特性的变化。结果：脑缺血再灌注后各时间点，ＰＭＮ与ＣＣＥＣ粘附力和粘附应力均明显高于正常对照组和伪手术组（Ｐ均＜０．０１）；加抗Ｍａｃ１抗体后，细胞粘附力和粘附应力均明显下降（Ｐ＜０．０５或Ｐ＜０．０１）。结论：脑缺血再灌注损伤后抗Ｍａｃ１抗体使ＰＭＮ与ＣＣＥＣ粘附力减小，粘附应力下降；

Objective:To investigate the potential mechanism underlying the protective effect of antimacrophage activating chemokine(Mac)1 antibody on ischemic neuron damage.Methods:Twentyfour Wistar rats were randomly divided into 4 groups:normal controls (n=4),sham operation group (n=4),cerebral ischemia group (n=8),and treatment group (antiMac1 antibody,n=8).Polymorphonuclear leukocytes (PMN) were separated from the blood ,and antiMac1 antibody was used to assay its inhibitory effect on adhesion between PMN and cultured cerebral capillary endothelial cells(CCEC) in vitro using micropipette aspiration technique after cerebral ischemiareperfusion.The mechanical properties of adhesion between PMN and CCEC cells were investigated in this study.Results:Following cerebral ischemiareperfusion,adhesion force and adhesion stress between PMN and CCEC were significantly elevated compared to normal controls and sham operation group (both P<001).Treatment with antiMac1 antibody,however,both adhesion force and adhesion stress were markedly reduced (P<005 or P<001).Conclusions:AntiMac1 antibody could reduce adhesion force and adhesion stress between PMN and CCEC after cerebral ischemiareperfusion damage.In view of its inhibitory effect on adhesion between PMN and CCEC cells,it is suggested that the application of antiMac1 antibody may be used in the treatment of ischemic cerebral vascular diseases.