草地早熟禾午夜2号植株再生研究

Study on Plant Regeneration from Midnight Ⅱ

以草地早熟禾(Poa pratensis L.)品种午夜2号成熟种子为外植体,进行植株再生研究,建立高频再生体系,为草地早熟禾进行原生质体培养和细胞融合奠定基础。结果表明:诱导愈伤组织的最佳培养基为MS+2,4-D(1mg/L)+6-BA(0.1 mg/L)+3%蔗糖+0.7%琼脂,其诱导率为52.3%;最佳继代培养基为MS+2,4-D(1 mg/L)+6-BA(0.3 mg/L)+3%蔗糖+0.7%琼脂;最佳分化培养基为MS+NAA(0.5 mg/L)+6-BA(1 mg/L)+3%蔗糖+0.6%琼脂、分化率为57.5%;生根培养基同分化培养基,供体材料经100 d的培养后获得再生植株。

In order to establish the basis for protoplast culture and cell fusion of Poa pratensis L. , the plant regeneration was studied using the mature seeds of Midnight II as explants. Results indicate that the optimal callus inducement medium was MS＋1 mg/L 2,4-D＋0.1 mg/L 6-BA＋3% sucrose＋0.7% agar and the callus inducement rate was 52.3 %; the best medium of subculture was MS＋1 mg/L 2,4-D＋0.3 mg/L 6-BA ＋3% sucrose＋0.7% agar; the best medium of differentiation was MS＋0.5 mg/L NAA ＋ 1 mg/L 6-BA ＋3% suerose＋0. 6% agar and the callus differentiation rate was 57.5%. The rooting medium was the same as the medium of differentiation. 100 d was needed to obtain the regenerated plantlet from donor materials.