多药耐药相关蛋白2、3的表达与食管鳞癌临床病理特征的关系

Relationship between expression of multidrug resistant associated protein 2,multidrug resistant associated protein 3 and clinical pathological features in esophageal squamous cell carcinoma

目的探讨多药耐药相关蛋白2(MRP2)和多药耐药相关蛋白3(MRP3)在食管鳞状上皮癌中的表达及其与食管鳞癌临床病理特征的关系及临床意义。方法应用免疫组织化学二步法检测68例食管鳞癌组织(分为高分化组30例和中低分化组38例)和30例癌旁正常黏膜组织(距离癌组织>2 cm)中MRP2和MRP3的表达情况。结果食管癌组织MRP2、MRP3的阳性表达率(72.1%和67.6%)均明显高于正常组织(40.0%和36.7%),两者在食管癌组织中的表达与浸润深度及淋巴结转移与否无关,与癌组织分化程度有关。MRP2在高分化组、中低分化组的阳性表达率分别为86.7%和60.5%(P<0.05);MRP3在高分化组、中低分化组的阳性表达率分别为83.3%和55.3%(P<0.05)。MRP2与MRP3在食管癌中的表达呈正相关。结论MRP2和MRP3可反映食管鳞癌的分化程度。食管鳞癌组织中MRP2和MRP3高表达可能是食管癌化疗效果不佳的重要原因之一,术后检测MRP2和MRP3不仅对临床化疗方案的选择具有重要意义,同时还可能对食管鳞癌预后的判断具有重要意义。

Objective To investigate the expression of multidrug resistant associated protein 2 （MRP2） and multidrug resistant associated protein 3 （MRP 3） in esophageal squamous cell carcinoma （ESCC） tissues and the relationship between their expression and clinical pathological features and clinical significance. Methods Two-pass immunhistochemistry was used to detect the expression of MRP2 and MRP3 in ESCC tissues of 68 patients （divided into high differentiated group, 30 cases and medium-lower differentiated group, 38 cases）, and surrounding noncancerous tissues （the distance beyond carcinoma tissues 2 cm） of 30 patients. Results The expressions of MRP2 and MRP3 were significantly higher in ESCC （72.1% and 67.6%） than those in normal tissues （40.0% and 36.7%）. The expressions were correlated with carcinoma differentiation,and not correlated with carcinoma invasive extent and lymph node metastasis in ESCC. MRP2 in high differentiated group and medium-lower differentiated group had a positive rate respectively 86.7% and 60.5%（P〈0.05） ; and MRP3 in the above groups was respectively 83.3% and 55.3%（ P〈0.05）. The expression of MRP2 in ESCC was positively related to expression of MRP3. Conclusion The expressions of MRP2 and MRP3 are associated with tumor cell differentiation. The high expression of MRP2 and MRP3 in ESCC may be one of the most important reasons of the poor effect of chemotherapy,postoperative detection is not only significant to the selection of chemotherapy schedule,but also to the judgement of ESCC prognosis.