维生素E抗大鼠实验性肝纤维化的作用

Effects of vitamin E on experimental hepatic fibrosis in rats

目的探讨维生素Ｅ（ＶｉｔＥ）治疗实验性肝纤维化的影响．方法用４００ｍＬ／ＬＣＣｌ４油剂皮下注射９ｗｋ诱导大鼠肝纤维化模型．将３０只肝纤维化大鼠随机分为ＶｉｔＥ治疗组（用ＶｉｔＥ１００ｍｇ／ｋｇ，ｉｖ，２次／ｗｋ，连续２０ｗｋ）、溶剂对照组及空白对照组．观察各组大鼠血清ＡＬＴ、ＡＳＴ、球蛋白（Ｇｌｂ）、丙二醛（ＭＤＡ）、脯氨酸肽酶（ＰＬＤ）及透明质酸（ＨＡ）的变化，并分别于治疗１０和２０ｗｋ取肝组织作病理检查及图象分析．结果大鼠肝纤维化模型形成时上述各项检测指标均显著异常，肝脏大量纤维组织增生．经ＶｉｔＥ治疗１０ｗｋ后，血清ＨＡ（μｇ／Ｌ，６７９±１１５降至３８８±１２９，Ｐ＜００１）显著下降，ＡＳＴ（ＩＵ／Ｌ，３０７±１２８降至６７±２９），Ｇｌｂ（ｇ／Ｌ，４９±６降至２８±６，Ｐ＜００５），ＭＤＡ（μｍｏｌ／Ｌ，１０４±３９降至４５±０９）及ＰＬＤ（Ｕ／Ｌ，４３４８±５７０降至２２１１±８８０）均恢复正常，溶剂及空白对照组各指标虽有下降（ＨＡ，μｇ／Ｌ，５７０±１８７和５９１±１２４，ＡＳＴ，ＩＵ／Ｌ，１３９±５７和１７０±３７，ＭＤＡ，μｍｏｌ／Ｌ，６１±１０和５７±０９，ＰＬＤ，Ｕ?

AIM To investigate the effects of vitamin E on hepatic fibrosis in rats induced by CCl 4. METHODS Liver fibrosis was induced in rats by subcutaneous injection of 400ml/L carbon tetrachloride oily solution for a period of nine weeks. The rats were randomly divided into treatment group ( n =10), solvent ( n =10) and blank control ( n =10) after the liver fibrosis model was established. The treatment group was treated with vitamin E (100mg/kg) twice a week for 10-20 weeks. Serum alanine transaminase (ALT), aspartate transaminase (AST), globulin (Glb), malondialdehyde (MDA) prolidase (PLD) and hyaluronic acid (HA) were determined periodically. Histopathological examination was made in the 10th and 20th week after the administration of Vit E. RESULTS All the serum parameters were obviously abnormal in rats with liver fibrosis. Proliferative fibrosis and pseudo lobules were observed in the liver. Serum HA (μg/L) was significantly decreased (from 679±115 to 388±129) after 10 week's vitamin E treatment. AST(U/L,from 307±128 to 67±29),Glb(g/L, from 49±6 to 28±6),MDA(μmol/L, from 10 4±3 9 to 4 5±0 9) and PLD(U/L,from 4348±570 to 2211±880) turned to normal. All the parameters of the solvent and blank control fell partly (HA,μg/L, 570±187 to 591±124,MDA,μmol/L,6 1±1 0 to 5 7±0 9,PLD,U/L, 3434±1147 to 4063±1274,AST,IU/L, 139±57 to 170±37) but still higher than that of the vitamin E group( P <0 01, P <0 05). Under light microscope, collagenous and reticular fibers in the liver tissue changed from a thick form to a slender one, some of which became shorter and broken. These changes were statistically significant as compared with that of the control groups examined by a real colour image analysis system ( P <0 01). CONCLUSION Vitamin E has good effects in the treatment of hepatic fibrosis induced by CCl 4 in rats.