siRNA介导的MKK4表达抑制研究

Study on the siRNA-mediated Silencing of MKK4 Expression

采用脂质体转染方法,将3对(siRNA ID#64447、siRNA ID#64539和siRNA ID#186583)人工合成的MKK4基因特异的小分子干扰RNA(siRNA)分别导入小鼠成肌细胞C2C12中。实时荧光定量PCR结果表明,转染了siRNA ID#64447的细胞,其MKK4 mRNA的表达水平下调得最多,约为85%。试验获得了能够高效下调MKK4基因表达的siRNA。

Mouse myoblast C2C12 cells were transfected with the synthesized MKK4-specific small interfering RNA （siRNA） du- plexes, siRNA ID# 64447, siRNA ID# 64539, and siRNA ]D# 186583, respectively, using Lipofectamine 2000. Real-time quantitative PCR results showed that the mRNA expression of MKK4 in C2C12 cells transfected with siRNA ID# 64447 was decreased approximately 85% , compared with cells transfected with the negative control siRNA. The siRNA which can effectively down-regulate MKK4 gene expression was obtained in this study.