三株牛源亚洲1型口蹄疫病毒VP1基因的克隆与序列分析

Cloning and Sequence Analysis of the VP1 Gene of Three Strains of Foot-and-mouth Disease Virus Type Asia 1

以3株国内分离的亚洲1型口蹄疫病毒(分别命名为F1、F2、F3)为研究目标,根据GenBank中注册的FMDVVP1基因的序列设计1对引物,采用RT-PCR方法成功地扩增出含有VP1全基因的片段,并测定了3个毒株VP1基因的序列。结果表明,3株亚洲1型FMDV毒株VP1基因长度均为633 bp,编码211个氨基酸。3株毒株彼此之间的核苷酸序列同源性在82.8%～99.1%之间,推导氨基酸序列同源性在89.1%～99.1%之间。从系统发生树看,F1株与我国香港2005年牛毒株序列同源性99.5%,属同一遗传谱系,F2株、F3株与2005年引起河北省万全县、北京市延庆县、甘肃静宁县疫情的毒株分属同一个基因群。

Three VP1 gene of three strains named F1, F2 and F3 of FMDV type Asial were amplified by RT-PCR respectively and PCR products were sequenced. The results suggested that the full length of FMDV serotype Asial VP1 gene all comprised 639 bp, coding for 213 amino acids. The nucleotide sequence homologies among 3 strains were between 82.8%-99.1% , and those of the amino acid sequence between 89.1%- 99. 1%. F1 strain was most closely related to strains isolated in Hong Kong in 2005. F2 and F3 strains were most closely related to strains isolated in Hebei, Beijing and Gansu in 2005 in China.