动胶菌发酵产物PHAs提取方法的研究

Study of the Method of Extracting PHAs from Zoologea sp. Batch Fermentation

以动胶菌为生产菌株,对后期提取方法进行筛选,从而确定了提取聚羟基烷酸酯(PHAs)的最佳方案即季铵盐浓度为1%,作用时间为15 min,细胞浓度为350 g/L,次氯酸钠浓度为3%,作用时间为5 min。以该方案为基础的放大实验,得到产物纯度98.3%;经力学性质测试拉伸强度为26.265 MPa;杨氏模量1287.535 MPa;经热分析测试熔点为173℃,熔解焓43.18Jg-1。

Experiment studied the servel methods of extraction poly hydroxyalkanoic acids （PHAs） from strain BRT. The product from industrial experiment purifacation rate was 98.3% , stress was 26. 626 MPa, the Young＇s modulus was 1287. 535 MPa, melting point was 173℃ ,melting ethalpy was 43.18Jg-1.