一株产糖纤维素分解青霉的分离及发酵条件优化

Screening of cellulolytic Penicillium and optimization of its fermentation conditions

从云贵高原地区次生林朽木中分离得到1株具有较高纤维素分解活性的青霉,PCR扩增18srDNA结果显示该菌属青霉。2%纤维素羧甲基纤维素钠、滤纸、脱脂棉、玉米叶等底物发酵结果表明该酶系具有较强的分解纤维素能力,不同温度下培养,粗酶活变化较大,以29℃最佳。

A cellulolytic Penicillium was screened from Yunnan-Guizhou plateau and identified by 18S rDNA PCR. Using 2% sodium carboxymethyl cellulose, filter paper, pledget and com leaf as substrate, the fermentation experiment indicated that the strain had a good ability of decomposing cellulose. The optimal fermentation temperature was 29℃.