摘要
实验室从新疆盐湖分离得到1株产α-淀粉酶中度嗜盐菌Bacillus sp．XJ1-05，对该菌株进行发酵条件优化和酶活性质研究，发现该菌株适宜的液态发酵工艺条件为：培养基碳源为酵母浸粉，氮源为大豆分离蛋白，两者比例1：1，培养温度33％，发酵时间72h，初始pH值7．0。最大酶活为98U，最佳反应温度为75℃，pH值为6．0-9．0。
The moderately halophilic bacteria Bacillus sp. XJ1-05 producing α-amylase was isolated from the salt lake of Xinjiang by the lab. The liquid fermentation conditions for α-amylase-producing and enzymology properties were studied. It was indicated that the optimal fermentation conditions were as follows： the carbon source was yeast extract; the nitrogen source was bean separation proteinum; the ratio of carbon source and nitrogen source was 1：1; initial pH7.0; 33℃ for 72h. The maxiumum enzymatic activity was 98U, the optimum temperature and pH of the enzyme were 75℃ and 6.0-9.0 respectively.
出处
《中国酿造》
CAS
北大核心
2009年第4期80-83,共4页
China Brewing
关键词
中度嗜盐菌
Α-淀粉酶
发酵条件
优化
酶活性
moderately balophilic bacteria
α-amylase
fermentation condition
optimization
enzymatic activity
