黄原胶发酵条件优化研究

Optimization of xanthan gum fermentation conditions

研究pH值、接种量、转速、发酵温度对黄原胶发酵的影响。通过摇瓶发酵,改变试验条件,测定发酵液黏度、粗胶含量来确定较为适合的发酵工艺。正交试验确定了黄原胶的最佳发酵条件为:pH7.2,接种量6%,转速200r/min,发酵温度28℃发酵72h。在此发酵条件下,产量达到25.5g/L,黏度为5569mPa·s。

In this paper, effects of pH value, inoculations, rotational speed, fermentation temperature on xanthan gum fermentation were studied. In shaken flask culture, the viscosity of fermentation broth, crude xanthan gum yield on different experiment conditions were determined for optimization. Orthogonal experimental analysis showed that the optimal fermentation conditions for producing xanthan gum were： initial pH 7.2, 6% inoculations, 200r/min, 28℃ for 72h. On this condition, xanthan gum yield was 25.5g/L, viscosity of fermentation broth was 5569cP.