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TNFAIP1基因通过抑制Bcl-2的表达诱导细胞凋亡 被引量：1

TNFAIP1 Induces Cell Apoptosis by Inhibiting Expression of Bcl-2
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摘要 将TNFAIP1基因成功克隆到真核表达载体pCMV-Myc中,研究TNFAIP1基因诱导细胞凋亡的机制.Hochest33258和流式细胞计数实验发现,过表达TNFAIP1能够诱导HeLa细胞凋亡.通过对凋亡相关基因的研究,发现过表达TNFAIP1能够抑制Bc l-2的mRNA水平和蛋白表达,推测TNFAIP1基因可能通过抑制Bc l-2的表达诱导细胞凋亡. To study the mechanism of TNFAIP1 induces cell apoptosis, TNFAIP1 is cloned into pCMV-Myc. HeLa cells apoptosis are observed using Hochest33258 staining and flow cytometry analysis. Further studies are taken for the mechanism of overexpressing TNFAIP1 induces cell apoptosis. RT-PCR and western blot analysis indicate that TNFAIP1 could inhibit the mRNA and protein level of Bcl-2. Those results elucidate a mechanism by which overexpressing TNFAIP1 induces cell apoptosis.
作者 孙振华 贺琼芝 颜峰 周畅
机构地区 湖南师范大学生命科学学院 教育部蛋白质化学与发育生物学重点实验室
出处 《湖南师范大学自然科学学报》 CAS 北大核心 2009年第1期112-115,共4页 Journal of Natural Science of Hunan Normal University
基金 国家自然科学基金资助项目(30500258)
关键词 TNFAIP1 Bd-2 细胞凋亡 TNFAIP1 Bcl-2 cell apoptosis
分类号 Q78 [生物学—分子生物学]
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参考文献10

  • 1WOLF F W, MARKS R M, SARMA V, et al. Characterization of a novel tumor necrosis factor-alpha-induced endothelial primary response gene[J]. J Biol Chem, 1992, 267:1 317-1 326.
  • 2LIN M C, LEE N P, ZHENG N, et al. Tumor necrosis factor-alpha-induced protein 1 and immunity to hepatitis B virus [ J ]. World J Gastroenterol, 2005, 11:7 564-7 568.
  • 3ZHOU J, HU X, XIONG X, et al. Cloning of two rat PDIP1 related genes and their interactions with proliferating cell nuclear antigen[ J ]. J Exp Zoolog A Com p Exp Biol, 2005, 303:227-240.
  • 4ZHOU J, REN K, LIU X, et al. A novel PDIP1-related protein, KCTD10, that interacts with proliferating cell nuclear antigen and DNA polymerase delta[ J]. Biochim Biophys Acta, 2005, 1729:200-203.
  • 5HE H, TAN C K, DOWNEY K M, et al. A tumor necrosis factor alpha- and interleukin 6-inducible protein that interacts with the small subunit of DNA polymerase delta and proliferating cell nuclear antigen[J]. Prec Nail Acad Sci USA, 2001, 98: 11 979-11 984.
  • 6杨利平,周爱冬,李红,张文峰,吴艳阳,张健,韩梅.人TNFAIP1基因在常见细胞系中的表达[J].遗传,2006,28(8):918-922. 被引量：7
  • 7HU W, XIE J, ZHAO J, et al. Involvement of BCL-2 family in apoptosis and signal pathways induced by cigarette smoke extract in the human airway smooth muscle cells [J/OL]. DNA Cell Biol, [2008-12-17 ]. http://www, nebi. nlm. nih. gov/ pubmedlg090673.
  • 8LESSENE G, CZABOTAR P E, COLMAN P M. BCL-2 family antagonists for cancer therapy[ J ]. Nat Rev Drug Discov, 2008, 7:989-1 000.
  • 9GROSS A, MCDONNELL J M, KORSMEYER S J. BCL-2 family members and the mitochondria in apoptosis[J]. Genes Dev, 1999, 13:1 899-1 911.
  • 10CATARZI S, MARCUCCI T, PAPUCCI L, et al. Apoptosis and Bax, Bcl-2, Mcl-1 expression in neutrophils of Crohn's disease patients[J]. Inflamm Bowel Dis, 2008, 14:819-825.

二级参考文献3

共引文献6

同被引文献9

  • 1杨利平,周爱冬,李红,张文峰,吴艳阳,张健,韩梅.人TNFAIP1基因在常见细胞系中的表达[J].遗传,2006,28(8):918-922. 被引量：7
  • 2HE H, TAN C K, DOWNEY K M. A tumor necrosis factor alpha--and interleukin 6-inducible protein that interacts with the small subunit of DNA polymerase delta and proliferating cell nuclear antigen [ J ]. Proc Natl Acad Sci U S A, 2001,98 : 11 979- 11 984.
  • 3LINK C D, TAFT A, KAPULKIN V, et al. Expression analysis in a transgenic caenorhabditis elegans alzheimer's disease model [J]. Neurobiol Aging, 2003, 24:397-413.
  • 4KAJIHO H, SAITO K, TSUJITA K, et al. RIN3,: a novel Rab5 GEF interacting with amphiphysin II involved in the early endocytic pathway [J]. J Cell Sei, 2003, 116:4 159-4 168.
  • 5SMITH T C, FANG Z, LUNA E J, et al. Novel interactors and a role for supervillin in early cytokinesis [ J ]. Cytoskeleton (Hoboken) , 2010, 67:346-64.
  • 6YOSHIKAWA M, KAJIHO H, SAKURAI K, et al. Tyr-phosphorylation signals translocate RIN3, the small GTPase Rab5- GEF, to early endoeytic vesicles [ J ]. Biochem Biophys Res Commun, 2008,372 : 168-72.
  • 7李红,贺志成,刘倩,周建林.建立可诱导表达TNFAIP1的稳定细胞系[J].湖南师范大学自然科学学报,2008,31(2):96-99. 被引量：1
  • 8钟英丽,刘云海,胡翔,肖顺勇,张健.用酵母双杂交筛选与AP-2α相互作用的因子[J].湖南师范大学自然科学学报,2003,26(3):73-77. 被引量：5
  • 9熊熙文,刘鑫,胡小晓,蒋小云,刘云海,张健.B12基因在小鼠部分肝切除后的表达差异分析[J].湖南师范大学自然科学学报,2004,27(1):71-74. 被引量：3

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湖南师范大学自然科学学报
2009年 第1期

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