雌激素受体α、β亚型在多囊卵巢综合征大鼠子宫内膜中的表达及意义

Expression and significance of estrogen receptor α and β in endometrium of rats with polycystic ovary syndrome

目的:探讨雌激素受体α、β亚型(Estorgen receptor α、Estrogen receptor β,ERα、ERβ)在多囊卵巢综合征(Polycystic ovary syndraome,PCOS)大鼠子宫内膜中的表达。方法:应用脱氢表雄酮(Dehydroepiandrosterone,DHEA)联合胰岛素构建PCOS大鼠模型(30只);采用放射免疫法测定大鼠血清性激素水平;免疫组化和RT-PCR技术检测ERα、ERβ蛋白及mRNA在PCOS大鼠和正常大鼠子宫内膜上的表达。结果:PCOS大鼠子宫内膜ERα蛋白的表达高于对照组(P<0.01);ERβ蛋白的表达两组无明显差异(P>0.05);RT-PCR检测显示ERα、ERβ mRNA在子宫内膜中的表达与免疫组化结果一致。结论:ERα在PCOS大鼠子宫内膜中的表达增高。

Objective： To determine the expression of estrogen receptor α and estrogen receptor β （ER α ,ER β ） in the endometrium of rats with polycystic ovary syndrome（PCOS ）, Methods： PCOS model was made by dehydroepiandrosterone （DHEA） and insuline （n=30）. The serum hormone levels were examined by radioimmunoassay. The expression of the mRNA and protein of ER α and ER β in endometrium of two groups were observed by using reverse transcription polymerase chain reaction （RT-PCR）and immunohistochemistry. Results： The expression of protein of ER α in PCOS group in endometrium was significantly higher than those in the control group（P〈0.01 ） ; whereas, the expression of protein of ER β in endometrium was not significantly different between the two groups（P〉0.05 ）. RT-PCR analysis showed the same expression of ER α mRNA and ER β mRNA in endometrium as immunohistochemistry. Conclusion： ER α in endometrium of PCOS rats is expressed excessively.