咪喹莫特诱导多品系小鼠脱毛动物模型的建立

Establishment of alopecia animal model induced by imiquimod in multi-strain mice

目的:研究咪喹莫特外用建立多品系小鼠脱毛动物模型的可行性及其初步机理。方法:BARB/c、129、C3H/HeJ、C574种品系小鼠各30只,各品系鼠均随机分成两组,实验组4组*15只,背部外用咪喹莫特每周3次;对照组4组*15只,外用不含药物的空白基质,随访一定时间后观察脱毛结果,并用免疫组化法检测毛囊周围TLR7受体的分布。结果:四种品系小鼠在给药7～10d后均出现脱毛现象,形成斑片状脱毛区,成功率100%。TLR7受体在各品系小鼠的毛囊及其周围均有强阳性表达。结论:咪喹莫特外用能稳定地诱导BARB/c、129、C3H/HeJ、C57品系小鼠建立脱毛动物模型,是一种非疤痕性、非炎症性脱发,其机理可能与咪喹莫特诱导激活毛囊周围的TLR7受体有关。

Objectives：To establish a novel alopecia animal model in muhi-strain mice. Methods：BARB/e mice, 129 mice, C57mice and C3H/HeJ mice were purchased at the age of 6 to 8 weeks. 30 mice of each kind were divided into control and experimental groups randomly. The control group were treated with vehicle alone. The experimental groups were treated with imiquimod cream with cotton swaps for back skin 3 times a week. The existence of TLR7 on skin was examined by immunohistoehemistry（IHC ）. Results：Alopecia lesions were observed in all the mice in experimental group, but mice in control group could not induce by imiquimod. The aggregation of TLR7 were observed in hair follicle. Conclusions： Irniquimod can stably induce non-inflammatory alopecia in BARB/c mice, 129 mice, C57mice and C3H/HeJ mice by activating TLR7.