一种大鼠异体肾移植模型的建立

Establishment of a rat model for orthotopic allograft kidney transplantation

目的:建立一种稳定,操作简便的肾移植模型。方法:以SD大鼠和Wistar大鼠分别作为供、受体。静脉用临时支架管,将供肾的下腔静脉与受体左肾静脉作端端吻合,供肾腹主动脉与受体腹主动脉端侧吻合,以及输尿管带膀胱瓣与膀胱吻合。结果:建立并改进了大鼠肾移植模型,手术总时间(130±16)min,热缺血时间小于20s,冷缺血时间小于50min,手术成功率90.77%。结论:此模型稳定、可靠,易于掌握。

Objective：To establish a feasible and stable rat model of kidney transplantation. Methods：SD and Wistar rats were used as the donors and the recipients, with the hellp of extradural catheter, the donor inferior vena was anastomosed end-to-end with the recipient left renal vein. An end-to-side anastomosis of abdominal aorta was operated. The donor ureter with ureterocystic flap was conducted to the recipient＇s bladder. Results：A modified rat model of kidney transplantation was successfully established. The average total operation time was（130 ± 20） min. The average time of warm ischemia and cold ischemia was less than 20 s and 50 min respectively. The success rate of operation reached about 90.77 %. Conclusion：This feasible model of kidney transplantalion is feasible and stable.