阿霉素脂质体的制备及对MCF-7/DOX作用的考察

Preparation of doxorubicin liposome and their effect on human MCF-7

目的:制备阿霉素(Doxorubicin,DOX)脂质体,并考察其对耐阿霉素的人乳腺癌细胞(MCF-7/DOX)的作用。方法:采用硫酸铵梯度法制备脂质体;采用MTT法考察DOX脂质体对MCF-7/DOX的细胞毒性和逆转多药耐药(Multydrug resistance,MDR)的效果。结果:阿霉素的平均包封率为90.77%。脂质体的细胞毒性实验结果显示,DOX脂质体的IC50比游离DOX小4倍;DOX脂质体对MCF-7/DOX的逆转倍数是游离DOX的4倍。结论:硫酸铵梯度法适用于制备DOX脂质体,其具有较好的细胞毒性作用及一定的逆转MCF-7/DOXMDR的作用。

Objective ： To prepare doxorubicin liposome and investigate its effect on human MCF-7 in vitro. Methods： DOX liposome was prepared by （ NH4 ）2SO4-gradient method.The MTT method was used to test cytotoxicity and reverse muhidrug resistance of DOX liposome on MCF-7/DOX. Results： DOX was encapsulated by 90.77%. The results of liposome MTT experiment suggested that IC50 of free DOX was 4 times bigger than DOX liposome.The DOX liposomes＇ reversing effect on MDR was 4 times as much as that of free DOX. Conclusion：The method of （NH4）2SO4 gradient is suitable for producing DOX liposome and it has better cytotoxicity and reversing MDR effect on MCF-7 than free DOX.