固相微萃取-气相色谱-质谱法分析毛泡桐花挥发性成分

Analysis of Volatile Compounds from the Flower of P. tomentosa by SPME-GC/MS

利用固相微萃取-气相色谱-质谱联用技术研究紫色毛泡桐花挥发性成分,探讨了不同纤维头取样、不同平衡温度、不同吸附和解吸时间等条件对分析检测结果的影响。结果表明,60℃下吸附35 min,PDMS/DVB纤维头能够有效地吸附紫色毛泡桐花的挥发性成分,其主要挥发性成分为茴香烯(25.88%)、3-辛酮(14.08%)、对甲氧基茴香醚(12.06%)、茴香酸甲酯(9.32%)、雪松烯(4.50%)、罗勒烯(4.34%)等醚类、酮类、酯类以及各种烯烃类化合物。

The volatile compounds from the flower of Paulownia tomentosa were obtained by solid phase micro-extraction-gas chromatography mass spectrometry （SPME-GC/MS）. The influence on detected data, involved sampling with different fibers and balancing samples in different temperature and time, was also discussed. The results show that the volatile compounds from the flower of Paulownia tomentosa can be effectively extracted by the fiber of PDMS/DVB at 60 ℃ for 35 min. The volatile compounds are some ethers, ketones, esters and some alkenes, mainly including anethole（25.88%）, 3-octanone （14.08%）, p-methoxy- anisole （12.06%）, 3-nonanone （13.59%）, 1,4-dimethoxy benzene （11.68%）, 4-methoxybenzoic acid methyl ester （8.85%）, cedrene （4.50%）, ocimene （4.34%）, and so on.