缺血预处理对大鼠缺血再灌注肾脏氧化亚氮合酶,L-选择素表达的影响

Effect of ischemic preconditioning on NOS and L-selectin expression in rat renal ischemic reperfusion

目的:观察缺血预处理后大鼠缺血再灌注肾脏氧化亚氮、氧化亚氮合酶水平和L-选择素表达的变化,探讨肾缺血预处理对肾缺血再灌注损伤的影响。方法:SD大鼠30只,分为假手术组、缺血再灌注组、缺血预处理组,于手术后2小时取血、肾组织检测氧化亚氮(NO)含量、氧化亚氮合酶(NOS)活性、超氧化物歧化酶(SOD)活性、丙二醛(MDA)活性,免疫组化法检测肾组织L-选择素(L-selectin)的表达水平。结果:缺血预处理组SOD活性明显高于缺血再灌注组(P<0.01),缺血预处理组MDA含量明显低于缺血再灌注组(P<0.01),缺血预处理组NOS,NO水平明显高于缺血再灌注组(P<0.01),缺血预处理组L-选择素表达明显低于缺血再灌注组(P<0.05)。结论:缺血预处理减轻肾缺血再灌注损伤的机制可能是增加NOS在血、肾脏的表达,抑制L-选择素的表达,进而维持肾SOD活力,减少MDA含量。

Objective： To observe the change of NO, NOS and L-selectin expression in rat renal ischemic reperfusion injury after renal ischemic preconditioning and investigate the effect of ischemic preconditioning on renal ischemic reperfusion injury. Methods： Thirty SD rats were randomly divided into three groups： sham-operated group, ischemic-reperfusion group, ischemic preconditioning group. The renal underwent 60-minute ischemic and 2-hour reperfusion. The level of Superoxide Dismutase （ SOD）, Malondial-dehyde （ MDA）, NO, NOS of kidney, blood in each group were examined, L-selectin expression in kidney was tested with immunohistochemichemistry. Results： SOD： The values in ischemic-preconditioning group were significantly higher than that in ischemic-reperfusion group （ P 〈 0.01 ）, MDA ： The values in ischemic-preconditioning group were significantly lower than that in ischemic-reperfusion group （ P 〈 0.01 ）, NOS, NO：The values in ischemic-preconditioning group were significantly higher than that in ischemic- reperfusion group. The expression of L-selectin in ischemic-preconditioning group was lower than that in ischemic-reperfusion group（P 〈 0.05 ）. Conclusion： Ischemic preconditioning could protect kidney from ischemic reperfusion injury, the mechanism might be that it increased NOS in kidneys and blood, so that the expression of L-selection was inhibited, with improving the SOD＇s activity and reducing the levels of MDA in kidney and blood.