脂肪变肝细胞中解偶联蛋白2表达的变化

Expression of uncoupling protein-2 in the normal hepatocytes and steatosis liver cells

目的:探讨解偶联蛋白2(UCP2)在正常肝细胞及不同程度脂肪变肝细胞中的表达情况。方法:用不同浓度的脂肪乳剂培养肝细胞,使其发生不同程度的脂肪变,作为脂肪变的肝细胞模型。正常细胞作为对照组,另设三组为模型组,其培养液内脂肪乳剂的浓度分别0.5,1.0,2.0μl/ml。应用RT-PCR技术检测细胞内UCP2 mRNA表达水平,蛋白质印迹检测UCP2的表达情况。结果:正常肝细胞内可以检测到UCP2 mRNA表达,且随着脂肪乳剂浓度的不断增加,脂肪变肝细胞内UCP2 mRNA相对表达量也增加。模型组与正常对照组表达量差异有统计学意义(P<0.01),模型组之间的表达量差异也具有统计学意义(P<0.01)。正常组内未检测到UCP2蛋白,模型组中随着肝细胞脂肪变程度的加重,UCP2蛋白表达也增加(P<0.05)。结论:脂肪变性的肝细胞中UCP2表达较正常肝细胞增加,且表达随脂肪变程度的加重而增加。

Objective ： To research the expression of uncoupling protein 2 in normal hepatocytes and fatty liver cells. Methods： Cultured hepatocytes with fatty emulsion, the cells got steatosis to varying degree and made them as the model of fatty liver cells. Normal cells used as control, cultured the model cells with medium containing different concentration of fatty emulsion as follows ：0.5,1.0,2.0 μl/ml. Testified the UCP2 expression by RT-PCR and Western blot. Results： We verified the normal cells contain UCP2 mRNA, and the higher the fatty emulsion, the more the UCP2 mRNA expression（P 〈 0.01 ）. There was no UCP2 protein expression in normal cells, and in model cells the UCP2 protein increased in accordance with the concentration of fatty emulsion（ P 〈 0.05 ）. Conclusion ： The expression of UCP2 in fatty liver cells was higher than normal ones, and there was a positive relationship between the steatosis of fatty liver cells and the quantity of UCP2.