中药脑脉Ⅱ号对脑缺血大鼠脑组织超微结构的影响

Effect of Naomai Ⅱ Injection on UItrastructure of Brain Tissues of Rats with Cerebral Ischemia

为了解以祛痰活血、开窍醒脑组方的脑脉Ⅱ号中药制剂对缺血的脑组织的影响，本实验将１４只大鼠分正常组、急性脑缺血模型组和脑脉Ⅱ号＋脑缺血组，脑缺血动物模型以结扎双侧颈总动脉合并血压下降法制备。电镜观察结果显示：正常组大脑皮层神经细胞、胶质细胞、毛细血管形态结构正常。模型组可见神经细胞肿胀，胞膜、核膜严重破损，胞质水肿，线粒体肿胀；胶质细胞病变严重；毛细血管管腔变窄，管壁粗糙。脑脉Ⅱ号＋脑缺血组神经细胞形态结构接近正常；胶质细胞轻度肿胀，胞质无明显水肿空白区域；毛细血管结构也基本正常。结果从组织形态学角度提示，祛痰活血、开窍醒脑中药能减轻脑缺血大鼠大脑皮层神经组织的损害。

Naomai Ⅱinjection is a herbal preparation with actions of promoting blood circulation, removing blood stasis and inducing resuscitation. This study is to explore its effect on cerebral ischemia: the rat model was established by ligating bilateral common carotid arteries and decreasing blood pressure. Fourteen rats were allocated to three groups: the normal control group(GroupⅠ),the acute cerebral ischemia model group (GroupⅡ) and the cerebral ischemia treated with Naomai Ⅱ injection group(GroupⅢ).The results observed with electron microscope were as follows: In GroupⅠ,the morphology of nerve cells, gliocytes and capillaries in the cerebral cortex was normal. In GroupⅡ,nerve cells were swollen, cytomembrane and nuclear membrane seriously damaged,cytoplasm edematous,mitochondrion swollen,gliocytes seriously deteriorated, lumen of capillary narrow and vessel wall roughened. In Group Ⅲ, the morphology of nerve cells was nearly normal, gliocytes slightly swollen, cytoplasm without obviously edematous blank spaces, and the structure of capillary essentially normal. It is indicated that NaomaiⅡ injection can reduce the damage of cerebral cortex in rats with cerebral ischemia, and can obviously protect cerebral tissues from ischemic injury.