HpD-PDT对人乳腺癌细胞增殖、凋亡的影响及作用机制

Impact of PDT on proliferation and apoptosis of human breast cancer cell line and its mechanism

目的:研究血卟啉衍生物(hematoporphrphyrin derivative,HpD)介导的光动力疗法(photodynamic therapy,PDT)对人乳腺癌细胞MDA-MB-231增殖、凋亡的影响,探讨其可能的作用机制,为HpD-PDT治疗乳腺癌提供理论依据。方法:体外培养的人乳腺癌细胞MDA-MB-231分为4组:A组(空白对照)、B组(单纯激光)、C组(单纯光敏剂)、D组(激光+光敏剂)。应用流式细胞术分析HpD-PDT对细胞周期的影响,免疫组织化学技术检测HpD-PDT对PCNA、Bax和Bcl-2蛋白表达的影响。结果:HpD-PDT作用后,乳腺癌细胞内G0/G1期细胞比显著高于对照组(P<0.05);PCNA阳性表达率明显低于对照组(P<0.01);Bcl-2蛋白的阳性表达率明显下降(P<0.05);Bax蛋白阳性表达率各组间均无统计学差异;实验组HpD-PDT作用后Bax/Bcl-2比值增高,呈时间依赖性变化。结论:HpD-PDT的作用机制可能与抑制肿瘤细胞增殖,诱导细胞凋亡有关。

Objective： To evaluate the effect of hematoporphrphyrin derivative （HpD） mediated photodynamic therapy （PDT） on proliferation and apoptosis of human breast cancer cell line MDA -MB -231, and to investigate the mechanism of its action. Methods ： MDA - MB - 231 cells were treated by 4 ways ： group A （ control ）, group B （laser）, group C （HpD）, group D （HpD -PDT）. Human breast cancer cells （MDA -MB -231 ） were treated with HpD - PDT in vitro. Cell cycle analysis was measured by flow cytometry （FCM） and the expressions of PCNA, Bax and Bcl - 2 were detected by immunohitochemistry. Results ： After HpD - PDT treatment, the cell number in G0/G1 phase increased （P 〈0.01 ） and in S phase decreased （P 〈0.05）. The expressions of PCNA and Bcl -2 in the experimental group were obviously lower than that in the control groups （P 〈 0.05 -0.01 ）. Bax protein expression was no differences among all groups （ P 〉 0.05 ）. The ratio of Bax/Bcl - 2 in the experimental group was higher than that in the control groups （ P 〈 0.01 ） , and it was significantly raised along with the time prolongation after PDT action. Condution：The mechanism of HpD - PDT may be related to inhibit human breast cancer cell proliferation and induce cell apoptosis.