乳腺癌细胞中核糖核酸酶抑制因子基因表达研究

Determination of the gene expression of ribonuclease inhibitor in breast carcinoma cells

采用反转录—聚合酶链式反应（ＲＴ—ＰＣＲ）和高效毛细管电泳（ＨＰＣＥ）法，对乳腺癌细胞中核糖核酸酶抑制因子（ＲＩ）ｍＲＮＡ含量进行检测。结果显示乳腺癌细胞中ＲＩｍＲＮＡ含量明显低于正常乳腺细胞中的含量，揭示乳腺癌细胞中ＲＩ基因表达水平低下，可能导致ＲＩ合成不足，对肿瘤细胞生长的抑制作用下降。

The mRNA content of ribonuclease inhibitor(RI)in breast carcinoma was determinated with RT-PCR and high performance capillary electrophoresis (HPCE)The result showed RI mRNA content in the carcinoma cells was remarkably lower than that of in the normal cells The areas of HPCE UV absorption of RT-PCR products between carinoma cells and normal ones were significantly difference(t test,P<001) This result suggested that the gene expresssion of RI in the breast carcinoma cells was reducing and RI synthesis was inefficient